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KMID : 0364820080440040305
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Korean Journal of Microbiology
2008 Volume.44 No. 4 p.305 ~ p.310
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Molecular Characterization of Clinically Isolated Staphylococcus aureus
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Oh Bo-Young
Kim Jung-Hee
Gong Young-Woo
Lee Jae-Mann
Go Jong-Myoung
Kim Yong-Hee
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Abstract
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Staphylococcus aureus is one of the most significant pathogens and a causative agents of nosocomial infections. The emergence of methicillin resistant S. aureus (MRSA), in particular, has become a major clinical and epidemiological problems worldwide. In this study, we analyzed the toxin genes and investigated molecular epidemiological characteristics of S. aureus isolated from stools of diarrheal patients at the hospitals in Incheon. Of the 609 strains from 2,281 specimens, 173 strains retained enterotoxin; 68 isolates (39.30%), 100 isolates (57.80%) were classified to A and C type, respectively. In the antibiotic susceptibility, all of enterotoxin positive isolates were resistant to oxacillin. Eighty eight strains (50.86%) of 173 MRSA isolate possessed tsst gene, but eta and etb genes were not detected at all. In the detection of MRSA associated genes by PCR method, mecA genes were detected in 167 strains (96.53%). From the result of PFGE analysis, we classified tsst-positive MRSA to 10 types and 24 subtypes. Type A, H and F were the major strains comprised of 57.95% (51strains), 10.22% (9 strains) and 9.09% (8 strains) respectively.
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KEYWORD
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antimicrobial resistance, MRSA, mecA, PFGE, tsst
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